Janitorial & Sanitation Reference Library · Technical Reference

Enzyme and Bio-Based Cleaners

Enzyme and bio-based cleaners use enzymes or live bacterial cultures to break down organic soils that cause odors and slow drains. This sheet explains how they work, where they help and what stops them from working.

Ref JS-0110

Draft, open for review. Check every value against the governing standard and the manufacturer’s data before use.

What it is

Enzymes are proteins that speed up the breakdown of specific soils. Each enzyme works on one type of soil. Bacterial (microbial) products contain dormant bacteria, often Bacillus spores, that become active in water and produce enzymes on site as they feed on organic soil. Enzyme products work quickly but stop when the enzymes are used up. Bacterial products work more slowly but keep working as long as the bacteria live. Both are used for urine odor, grease traps, drains, carpet spills and organic stains.

How to identify

The label describes the product as enzymatic, bio-enzymatic, microbial or bacterial, and often lists the soils it targets. Bacterial products may list a count of bacteria and a shelf life. Many are near neutral pH, have little or no fragrance, and are not EPA-registered disinfectants.

Enzymes and their soils

Enzyme or cultureBreaks downTypical use
ProteaseProteinsBlood, food, body soils, urine-related soils
LipaseFats and oilsGrease traps, kitchen drains
AmylaseStarchesFood spills, sauces
CellulaseCellulosePaper and fiber buildup in drains
Bacterial culturesMixed organic soils, including urine residuesOngoing odor control, restrooms, drains, grease traps

How to use it

  1. Remove as much soil as possible first.
  2. Apply enough product to reach all of the soil, including into carpet backing or grout.
  3. Allow the dwell time on the label. Enzymes and bacteria need time and moisture. Many products work best left overnight.
  4. Keep the area damp if the label says so. Drying stops the action.

Key points

  • Disinfectants, bleach, quats, strong acids and strong alkalis kill bacteria and can destroy enzymes. Do not use them on the same spot right before or after a bio product.
  • Very hot water can damage enzymes and bacteria. Follow the label's temperature guidance.
  • Bio products are for odor and soil removal, not for killing germs. They do not replace disinfection where it is required.
  • Drain and grease trap treatments work best on a regular schedule, usually when flow is low, such as at night.

Common mistakes

  • Spraying a disinfectant over an enzyme treatment and wondering why the odor came back.
  • Wiping up an enzyme product right after applying it.
  • Using hot water to dilute a bacterial product.
  • Expecting a bio product to clear a fully blocked drain. Clear it mechanically first.

Governing standards: No single governing standard. Follow the product label and SDS.

Reference only. Verify against the current governing code or standard, the manufacturer's data and the project documents before use. Apps for Distributors disclaims liability arising from reliance on this reference.

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